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Class II BSCs differ from Class I cabinets in that they allow only air from a HEPA-filtered (sterile) supply to flow over the work surface.
A Class II type A2 BSC is shown in Figure 2. An internal fan draws room air (supply air) into the cabinet through the front opening and then into the front intake grill. After passing through the grill, the supply air is drawn upwards and through a HEPA filter before flowing downwards over the work surface.
In general TB laboratories that perform diagnostic tests, an autoclave that uses saturated steam under pressure is the most efficient means of sterilizing instruments, glassware and media solutions; it is also used for decontaminating biological material (such as mycobacterial cultures). Two factors are essential for an autoclave to function optimally: (1) all of the air in the chamber should be replaced by steam; and (2) the temperature must be 121 °C.
In a laboratory where there is a moderate risk of infection, there are two levels of containment: the BSC (primary containment) and the laboratory itself (secondary containment). To address the specific risks associated with a moderate-risk laboratory, the following mitigation and control measures should be established.
TB laboratory facilities can be classified into three main levels of procedural risk, based on the activities being performed and their associated risks:
Risk is the combination of the likelihood that a specific hazard will be encountered and the consequences of an event related to that specific hazard. Risks should be identified and categorized, and a determination should be made about which risks need to be controlled or minimized. The analysis of aerosolization risks described in this manual has led to the development of minimum biosafety requirements necessary for performing different procedures in TB laboratories.
Personal protective equipment and clothing may act as barriers to minimize the risk of exposure to aerosols, splashes and accidental inoculation. The choice of clothing and equipment depends on the nature of the work. Protective clothing should be worn whenever staff work in the laboratory (see Box 6). Before leaving the laboratory, staff should remove their protective clothing, and wash their hands. Table 5 summarizes the types of personal protective equipment used in laboratories and the protection each type offers.
Table A6.1 proposes a list of variables on which data should be collected for index cases and their contacts in an evaluation of the contacts of a person with TB disease. The data may be collected at various stages of the investigation. Usually, demographic information and much of a person’s medical history is available at the first visit, while other details, such as the results of tests for ruling out TB, confirming infection or starting TPT would be collected at subsequent encounters.
Background: A programme review is an integral part of the management cycle of a TB programme. It is a structured way of assessing the performance of the programme and for improving its quality; it is also the basis for developing or updating the national strategic plan. PMTPT is one of several preventive actions envisaged in the End TB Strategy, such as TB case-finding, infection control, prevention and care of comorbidities (e.g. HIV), access to universal health care, social protection and poverty alleviation.
